If your web browser automatically blocks the installation file, select "Keep" to recognize it as a trusted file.
StereoMap 4.2 highlights:
Visual Explore:
When the number of spots is too large (bin1, or bin5 on chips larger than 2×2 cm), the software automatically reduces the displayed spots to accelerate rendering. You can adjust the reduction ratio or disable this behavior in Layer Menu > Display Options > Level of Detail in heatmap view settings.
Image Processing:
Step 1: No need to enter microscope parameters when uploading QC stitched images.
Step 2: Added matrix transparency adjustment for morphological registration.
Step 4: Added newautomatic model segmentation option for cell segmentation.
Added dashed box to indicate valid spatial matrix area in Steps 2, 3, and 4. Note: regions outside the box will not be recorded.
Tools:
Image QC no longer requires microscope parameters for stitched images.
In DAPI + mIF QC, calibration evaluation results are no longer a deciding factor for QC pass/fail.
Removed QC adaptation for multi-channel fluorescence images.
For large dataset analysis (100K+ cells/bins), 32 GB RAM and a quad-core processor are highly recommended for optimal performance.
When working with external storage devices (e.g., external hard drives), performance may be affected due to slower I/O speeds, increased latency, and potential crashes due to unexpected disconnection.
Installation
StereoMap for Windows is distributed as a self-installing executable (.exe) file. Double-click on the installation file to start installing.
If you encounter the following prompt during the installation, you can click "Open"，and add it to the trusted file.
Follow the prompts to choose an installation directory. The default directory is the local D drive.
Before you install, verify that there is enough disk space in the target directory. For both new installation and upgrade, your installation directory must meet the minimum of 20 GB of available disk space.
Avoid installing the application in a directory path that has special characters or symbols. The supported characters include space, underscore, letters A - Z, and numbers 0 - 9.
Re-run Image QC to convert the compressed TAR (.tar.gz) image file to the latest version.
Setting
Application Update
Click on the setting gear at the top right of the window to open the Setting page. Select About to get the version information and check the updates manually (internet required). If a new version is available, you will be asked to download and install a new package.
You can turn on the auto-update option so that the application will check the new versions automatically in the background.
Saving Path
The default saving path is your installation directory, you may change it to your preferred location from Setting as shown below.
at the top right of the window to open the Setting page. Select About to get the version information and check the updates manually (internet required). If a new version is available, you will be asked to download and install a new package.
